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gene deletion 【生物學】基因刪除。

gene insertion

Shaking flask experiments and hplc analyses showed that the four mutants no longer produced the toxic oligomycin , and only made four components of avermectins b , which were avermectin b1a , b1b , b2a , b2b . the yields of avermectins b in these mutants were separately equal to those in cz8 - 73 . this revealed that olma genes deletion did n ' t affect the biosynthesis of avermectins 將4株經southern雜交驗證正確的基因缺失突變株進行搖瓶發酵和hplc檢測,發現4個突變株均不再產生寡霉素而僅產阿維菌素b組分,阿維菌素的總產量和b1的產量與出發菌株相當,說明寡霉素pks基因簇的缺失并不影響阿維菌素的生物合成。

In addition to avermectins , s . avermitilis produces oligomycin , a strongly toxic compound . gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73 , the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin . olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover 本研究以產阿維菌素b和寡霉素的阿維鏈霉菌cz8 - 73為出發菌株,構建了基因缺失載體pxl05 ,并將其轉入cz8 - 73中,通過缺失載體和染色體之間的同源雙交換,對染色體上長達90kb的寡霉素聚酮合酶( pks )基因簇( olma )進行了缺失。

2 . the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein , encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids . 19 highly conserved , potential glycosylation sites and 17 cysteines residues were characterized with si protein , homology analysis showe that there were gene deletion - S1基因:其全序列共1614bp (從起始密碼子atg到s前體蛋白裂解位點) ,編碼537個氨基酸,其氨基端有一編碼18個氨基酸的信號肽序列,第12 13位氨基酸殘基構成了信號肽的切割位點, 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。

Analysis of dna microarray assay of progressive muscular dystrophy gene deletions 微陣列對進行性肌營養不良基因缺失檢測的分析

Effect of avec gene deletion on avermectins production in streptomyces avermitilis 基因缺失對產素調控的影響

Gene deletion to decrease acetic acid yield of saccharomyces cerevisiaes 6基因降低啤酒酵母產乙酸量